Characterization of individual cell motion in human skin capillaries by noninvasive reflectance confocal video microscopy (Conference Presentation)

Inga Saknite; Michael Byrne; Eric R. Tkaczyk

doi:10.1117/12.2510442

11 March 2019 Characterization of individual cell motion in human skin capillaries by noninvasive reflectance confocal video microscopy (Conference Presentation)

Inga Saknite, Michael Byrne, Eric R. Tkaczyk

Author Affiliations +

Proceedings Volume 10877, Dynamics and Fluctuations in Biomedical Photonics XVI; 108770D (2019) https://doi.org/10.1117/12.2510442
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Leukocyte-endothelial interactions have been well-characterized by intravital microscopy in mice. Quantitative parameters descriptive of these dynamic processes, e.g. the level of leukocyte rolling, adhesion and extravasation, can detect and track inflammation. Despite technology available to study individual cell motion noninvasively in human skin, we are not aware of any published exploratory or clinical studies. In this preliminary study, we explore the feasibility to extract parameters characteristic of individual cell motion in the postcapillary vessels of healthy human skin from videos taken by a noninvasive clinical confocal microscope (Vivascope 1500). The microscope is capable of real-time imaging of individual cells at 9 frames per second. We took videos of ten cutaneous vessels per each of two body sites (volar forearm and upper anterior chest) of ten healthy subjects. We then characterized the dynamic motion of cells via subsequent video analysis by extracting the following parameters: blood flow velocity, number of adherent leukocytes (stationary <30 s), and number and diameter of vessels. We observed variation in blood flow velocity within 1 minute in the same vessel, between vessels within an 8x8 mm field of view, and within two different body sites. Leukocyte adhesion, more commonly associated with inflammatory conditions, can also be observed in healthy skin. Further studies are needed to test the potential of this approach to detect inflammation.

Conference Presentation

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Inga Saknite, Michael Byrne, and Eric R. Tkaczyk "Characterization of individual cell motion in human skin capillaries by noninvasive reflectance confocal video microscopy (Conference Presentation)", Proc. SPIE 10877, Dynamics and Fluctuations in Biomedical Photonics XVI, 108770D (11 March 2019); https://doi.org/10.1117/12.2510442

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KEYWORDS

Skin

Confocal microscopy

Capillaries

Reflectivity

Video microscopy

Video

Blood circulation

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